TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| Escherichia coli strains | ||
| XL-1 Blue | supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac[F′ proAB+lacIqlacZΔM15 Tn10 (Tetr)] | Stratagene |
| MC1061 | araD139 Δ(ara leu)7697 ΔlacX74 galU galK hsdR2 strA mcrA mcrB1 | 7 |
| TH201 | K-12 ΔzntA ΔzitB | This study |
| Pseudomonas putida strains | ||
| KT2440 | Wild type | 2 |
| KT2440.1 | KT2440 ΔczcA1 | This study |
| KT2440.2 | KT2440 ΔczcA2 | This study |
| KT2440.3 | KT2440 ΔcadA1 | This study |
| KT2440.4 | KT2440 ΔcadA2 | This study |
| KT2440.21 | KT2440 ΔczcA2 ΔczcA1 | This study |
| KT2440.23 | KT2440 ΔczcA2 ΔcadA1 | This study |
| KT2440.24 | KT2440 ΔczcA2 ΔcadA2 | This study |
| KT2440.14 | KT2440 ΔczcA1 ΔcadA2 | This study |
| KT2440.13 | KT2440 ΔczcA1 cadA1::FRTkan | This study |
| KT2440.34 | KT2440 ΔcadA1 cadA2::FRTkan | This study |
| KT2440.241 | KT2440 ΔczcA2 ΔcadA2 ΔczcA1 | This study |
| KT2440.243 | KT2440 ΔczcA2 ΔcadA2 ΔcadA1 | This study |
| KT2440.143 | KT2440 ΔczcA1 ΔcadA2 cadA1::FRTkan | This study |
| KT2440.231 | KT2440 ΔczcA2 ΔcadA1 czcA1::FRTkan | This study |
| KT2440.2431 | KT2440 ΔczcA2 ΔcadA2 ΔcadA1 czcA1::FRTkan | This study |
| Plasmids | ||
| pKD4 | Ampr, oriR[R6Kγ], FRT-kan-FRT | 9 |
| pFLP2 | AmprsacB, encodes the Flp recombinase | 14 |
| pEX18Tc | Tcr, oriT sacB, gene replacement vector with multiple-cloning site from pUC18 | 14 |
| pSL1190 | Ampr, cloning vector | Amersham Pharmacia Biotech |
| pSLlux | Ampr, luxCDABE in pSL1190 | 19 |
| pDN18N | Tcr, T7 expression vector, RK2 replication origin | 26 |
| pEXcadA1FRTkan | Tcr Kmr, fragment of cadA1 gene interrupted by FRT-kan-FRT in pEX18Tc | This study |
| pEXcadA2FRTkan | Tcr Kmr, fragment of cadA2 gene interrupted by FRT-kan-FRT in pEX18Tc | This study |
| pEXczcA1FRTkan | Tcr Kmr, fragment of czcA1 gene interrupted by FRT-kan-FRT in pEX18Tc | This study |
| pEXczcA2FRTkan | Tcr Kmr, fragment of czcA2 gene interrupted by FRT-kan-FRT in pEX18Tc | This study |
| pDNPcadA1lux | Tcr, 682-bp fragment from cadA1 operator area in front of luxCDABE operon cloned into vector pDN18N | This study |
| pDNPcadA2lux | Tcr, 93-bp fragment from cadA2 operator area in front of luxCDABE operon cloned into vector pDN18N | This study |
| Tcr, 409-bp fragment from czcCBA1 operator area in front of luxCDABE operon cloned into vector pDN18N | This study | |
| pDNPczc2lux | Tcr, 991-bp fragment from czcCBA2 operator area in front of luxCDABE operon cloned into vector pDN18N | This study |
| pDNlux_2 | Tcr, luxCDABE in pDN18N opposite the T7 promoter | This study |
| pTIHis | Ampr, 5-kb PCR product of pFLP2 containing rightward λ promoter, cI857, and ori1600, self-ligated | This study |
| pTIcadA1His | Ampr, cadA1 in pTIHis | This study |
| pTIcadA2His | Ampr, cadA2 in pTIHis | This study |
| pTIczcA1His | Ampr, czcA1 in pTIHis | This study |
| pTIczcA2His | Ampr, czcA2 in pTIHis | This study |
| pTIczcCBA1His | Ampr, czcCBA1 in pTIHis | This study |
| pTIczcCBA2His | Ampr, czcCBA2 in pTIHis | This study |
| pTIluxHis | Ampr, luxCDABE in pTIHis | This study |