TABLE 2.
Sequences of the primers used for PCR amplification
| Primer pair | Sequence (5′-3′)a |
|---|---|
| A1 | ACTTAGGCATGCGTTGGAAGCGGTCCGTGAAT |
| ACTTAGAAGCTTGCTTGAACGCCCTGATGACC | |
| A2 | AGTGGTGAATTCGAGCCCTCATGGATCGCAAC |
| AGTGGTGAGCTCTCGCTGTGGAGTCGATGAGC | |
| A3 | AGTTGAGAGCTCCGCTCATGGTGATGACGACC |
| AGTTGAGAGCTCCTTCTTCGCCGCCTGGGTGA | |
| A4 | ACTTAGGAATTCATGACCAGCAGCAAGGCGAC |
| GCAAAGCTTCTGCCGAACAC | |
| A5 | AGTTGAGAGCTCGGCAGCTTTGTCCTTAAGTC |
| AGTTGAGAGCTCGGCAGAAATAGACAGCGGAA | |
| A6 | ACAACCTCCTTAGTACATGCAACC |
| CATCATCATCATCATCATTAACCCTGGCGTTACCCAACT | |
| A7 | ATGAAGAGTTTGCTTGAACG |
| GCTTCTGCGGTAGGCCAACA | |
| A8 | ATGAACCAGCCTGTCAGCCA |
| CAAAAGGCGCAAGCCGTTGA | |
| A9 | ATGTTTGAACGTATCATCAGATTCGCCA |
| AACCACTTCGGCCTCGTCGC | |
| A10 | CGGGGACACCTGCATGTTCG |
| CTGCTCATCCCGCCGATACG | |
| A11 | CAAAGTGCCCCGGTATAACC |
| AACCACTTCGGCCTCGTCGC | |
| A12 | ATGCTTCGCCCAGAGGACTGT |
| CTGCTCATCCCGCCGATACG | |
| A13 | CCGCTAAAGAGAAGCCCACA |
| AACTCGTCCCTTCTGGGGTC | |
| A14 | AGTACCTGCAGCACGAAATCTCCAGCAAGTG |
| CAAGTGGATCCGGGGTCATCCTTAAATTGAG | |
| A15 | CCTTACTGCAGATGCTCCTTTGATTCACGGC |
| ACTTCGGATCCTGATGGTCCTCGTGCACAAA | |
| A16 | CTTGCCGTCCTTGAGCACCA |
| GCCAGGGACTGTAGAAAGGG |
a
Underlining indicates restriction sites.