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SDS-PAGE analysis of the recombinant chromate reductase from Thermus scotoductus SA-01 expressed in E. coli. Lane 1, standard molecular mass marker; lane 2, soluble fraction (10 μg) of E. coli transformed with pET22b(+); lane 3, soluble fraction (10 μg) of E. coli transformed with pET22b(+) containing the chromate reductase gene; lane 4, soluble fraction (1 μg) after heat denaturation of host proteins. Proteins were visualized by Coomassie blue staining.
