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Summary of melting temperatures of WT and substituted LUSH proteins determined by circular dichroism. In all cases the concentration of butanol was 80 mM.
| TM (°C) Apo | TM (°C) + butanol | Δ TM (°C) | |
|---|---|---|---|
| Wild-Type | 49.0 ± 0.2 | 56.0 ± 1.8 | 7.0 |
| T57S | 49.1 ± 1.9 | 56.4 ± 1.2 | 7.3 |
| S52A | 58.2 ± 1.1 | 61.7 ±1.9 | 3.5 |
| T57A | 53.4 ± 2.3 | 54.2 ± 2.4 | 0.8 |
