Table 2.

Primers used for quantitative PCR of mouse FMO and housekeeping genes.

Gene	Primer Sequence (5’ to 3’)	Exon	Amplicon Size (bp)	Annealing Temperature	ENSEMBLE Transcript1	Reference
Fmo1	5’-TGTCTCTGGACAGTGGGAAGT	4	217	56	ENSMUST00000046049	[26]
	5’-CATTCCAACTACAAGGACTCG	5
Fmo2	5’-AGGCTCCATCTTCCCAACCGTA	7	382	56	ENSMUST00000045902
	5’-CCGGGTCTTTAAGGGTTTCAGG	9
Fmo3	5’-CACCACTGAAAAGCACGGTA	4	445	58	ENSMUST00000028010	[26]
	5’-GTTTAAAGGCACCAAACCATAG	6
Fmo5	5’-ATCACACGGATGCTCACCTG	4	235	58	ENSMUST00000029729	[26]
	5’-GCTTGCCTACACGGTTCAAG	6
Hprt1	5’-AGCTACTGTAATGATCAGTCAAC	3/4	180	53	ENSMUST00000026723	RT Primer DB ID:502
	5’-AGAGGTCCTTTTCACCAGCA	7
Rpl13a	5’-GCTCTCAAGGTTGTTCGGCTGA	6	157	53	ENSMUST00000102669
	5’-AGATCTGCTTCTTCTTCCGATA	7
Tbp	5’-GGCCTCTCAGAAGCATCACT	2/3	167	53	ENSMUST00000039079	RT Primer DB ID:492
	5’-GCCAAGCCCTGAGCATAA	3
Actb	5’-CTACAGCTTCACCACCACAG	4	137	62	ENSMUST00000031564
	5’-GCAGCTCATAGCTCTTCTCC	4

¹http://www.ensembl.org/index.html

²http://medgen.ugent.be/rtprimerdb/