Figure 1. Decreased neointima formation after carotid artery ligation in Rock1^+/– mice.

(A) Representative cross sections of contralateral unligated and ligated carotid arteries in WT, Rock1^+/–, and Rock2^+/– mice at 28 days after ligation. Scale bar: 100 μm. (B) Representative immunohistochemical analysis of ROCK expression and activity in carotid arteries from WT and Rock1^+/– mice at 14 days after ligation. Vessels were stained with nonspecific antibody (IgG), and antibodies were directed at total MBS (tMBS), phosphorylated MBS (pMBS), ROCK1, and ROCK2. The I and M indicate the intima and media, respectively. Scale bar: 50 μm.