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. 2008 Apr 15;118(5):1632–1644. doi: 10.1172/JCI29226

Figure 4. Decreased recruitment and adhesion of leukocytes in Rock1+/– mice.

Figure 4

Neutrophil and macrophage recruitment was induced by i.p. injection of thioglycollate. (A) Neutrophil recruitment 4 hours after i.p. injection of PBS (control) or 3% thioglycollate into WT, Rock1+/–, and Rock2+/– mice (n = 10, 5, and 5, respectively). (B) Macrophage recruitment 4 days after i.p. injection of PBS (control) or 3% thioglycollate into WT, Rock1+/–, and Rock2+/– mice (n = 9, 8, and 6, respectively). Confluent monolayers of ECs isolated from WT and Rock1+/– mice were incubated with PBS (control, n = 4–6 in each group) or 10 units/ml of thrombin for 10 minutes (n = 9–10 in each group). They were then placed under laminar flow at 0.5 (C) or 1.0 dynes/cm2 (D). U937 monocytes, stably expressed human L selectin (U937-LAM), were perfused over the EC monolayers, and adherent cells were quantified after 3 minutes. *P < 0.01 versus each control group; †P < 0.05 versus each control group; ‡P < 0.01 versus Rock1+/– mice with thioglycollate or Rock1+/– ECs with thrombin; ΧP < 0.05 versus Rock1+/– mice with thioglycollate or Rock1+/– ECs with thrombin.