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. 2008 Apr 15;118(5):1632–1644. doi: 10.1172/JCI29226

Figure 7. Decreased cell proliferation in the neointima of Rock1+/– mice.

Figure 7

(A) Representative histological sections from carotid arteries in WT and Rock1+/– mice stained for PCNA at 14 days after ligation. Arrowheads indicate PCNA-positive cells. Scale bars: 50 μm (left panels). Quantitative analysis of the ratio of PCNA-positive cells to total cell number in the intima and the media (n = 10–16) (right panel). *P < 0.01 versus WT mice. (B) Cell proliferation in response to serum of VSMCs from WT and Rock1+/– mice. Experiments were performed 6 times in triplicates. (C) DNA synthesis in response to PDGF of VSMCs from WT and Rock1+/– mice (n = 12). *P < 0.01 versus without PDGF (control). (D) Cell migration in response to PDGF of VSMCs from WT and Rock1+/– mice (n = 8­–9). *P < 0.01 versus without PDGF (control); †P < 0.01 versus control; ΧP < 0.01 versus WT. (E) Representative western blot analysis of ROCK and ERK activities in VSMCs of WT and Rock1+/– mice. VSMCs were stimulated with 10 ng/ml of PDGF for the indicated time periods.