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Experimental design. (A) When the Δα and Δω β-gal mutants are fused to proteins that do not dimerize, their association is not favored and β-gal activity is not detected. (B) When the Δα and Δω β-gal mutants are fused to proteins that can dimerize, the formation of active β-gal is favored. (C) Schematic representation of the FKBP12-Δω-Neo and the FRAP-Δα-Hygro constructs. IRES, internal ribosome entry sequence; LTR, long terminal repeat.
