Figure 2.

Biochemical assay of induction of β-gal activity upon chimera complementation. (A) Kinetics of induction of β-gal activity upon treatment with rapamycin. Pure populations of C2C12 cells stably expressing both FKBP12-Δω and FRAP-Δα were plated in 96-well plates and 10 ng/ml rapamycin was added at time zero. Cells were then lysed at different time intervals thereafter, and the β-gal activity in the lysates was quantitated by chemiluminescence. (B) Dose response of β-gal activity upon rapamycin treatment. C2C12 cells expressing both FKBP12-Δω and FRAP-Δα were plated in 96-well plates and treated with different concentrations of rapamycin for 3.5 hr. β-gal activity is expressed as luminescence counts per second. Each point represents the average of six replicate samples. Error bars indicate standard deviations from the mean.