TABLE 3.
Sensitivities of rtV173L mutants to lamivudine, adefovir, and penciclovir
| HBV mutant(s) | Lamivudine
|
Adefovir
|
Penciclovir
|
|||
|---|---|---|---|---|---|---|
| IC50 (μM)a | Fold resistanceb | IC50 (μM) | Fold resistance | IC50 (μM) | Fold resistance | |
| None (wild type) | 0.04 ± 0.01 | 1.0 | 0.23 ± 0.21 | 1.0 | 65.34 ± 34.0 | 1.0 |
| rtV173L | 0.03 ± 0.01 | 0.8 | 0.18 ± 0.12 | 0.8 | 76.2 ± 6.6 | 1.2 |
| rtL180M + rtM204V | >100 | >2,500 | 0.20 ± 0.04 | 0.9 | >400 | >6 |
| rtV173L + rtL180M + rtM204V | >100 | >2,500 | 0.12 ± 0.05 | 0.5 | >400 | >6 |
a
IC50 values were calculated by nonlinear regression of antiviral data generated by Southern blotting (Fig. 2) and PhosphorImager analysis and represent the mean and standard deviation of three or more independent experiments.
b
Fold resistance was calculated as the ratio of mutant IC50 to wild-type IC50.