FIG. 3.

Sequence comparison of MV genomes. (A) Nucleotide changes for each coding region (capital letters in boxes) and in noncoding regions (lowercase letters) are shown in the lower part. Amino acid changes are shown in the upper part (one-letter amino acid symbol). The nucleotide (nt) and amino acid (aa) changes that are present only in the EdB-tag sequence are highlighted in gray. Nucleotide changes in positions 1805 and 1806 of EdB-tag correspond to the tag introduced. (B) Phylogenetic tree showing the EdB-tag among the Edmonston group (24) and two wild-type isolates (31, 32). The sequences were aligned using Clustal W (34). Nucleotide sequence distances were determined with Dnadist of the Phylip package, version 3.5 (9). The tree was derived by neighbor-joining analysis applied to pairwise sequence distances calculated by using a variety of methods, including the Kimura two-parameter method to generate unrooted trees. The final output was generated with Treeview (21).