TABLE 1.
Oligonucleotide primers used in this study
| Primer or oligonucleotide | Sequence |
|---|---|
| Primersa | |
| m41-HA | 5′-aaa gga tcc acc atg gga gac gat gat cgt c-3′ |
| 5′-aaa gaa ttc aAG CGT AGT CTG GGA CGT CGT ATG GGT Atc tgt caa tga tca cga cga-3′ | |
| HA-m41 | 5′-aaa gga tcc acc atg TAC CCA TAC GAC GTC CCA GAC TAC GCT atg gga gac gat gat cgt c-3′ |
| 5′-aaa gaa ttc tca tct gtc aat gat cac gac-3′ | |
| UL37x1-HA | 5′-aaa gaa ttc cac cat gtc tcc agt cta cgt gaa t-3′ |
| 5′-aaa ctc gag tca AGC GTA GTC TGG GAC GTC GTA TGG GTA ctg gtg aga ctg ctg ggg | |
| Oligonucleotidesb | |
| MCMVΔm41 | 5′-AAT AGT CAT CCG ATG ATC GTG TCG CCG CCC GAC CGC CCT CCT CCC CCA ATt gtt gac aat taa tca tcg gca t-3′ |
| 5′-CGC CGT TTC CTC ACA TTC CGT TGT CGT GCG CAG GTT CCT CCG AAC CTT TGt cag tcc tgc tcc tcg gcc a-3′ | |
| MCMVΔm40 | 5′-ACC ATC CCC TCG ACT CGC GTC TTC TCT GTC GAT CTG GGT GTC GGG GAG GGt cag tcc tgc tcc tcg gcc a-3′ |
| 5′-TAG CTA TCA CTT TCG AAA CGC ATA AAC GAC GCT ATG TCC CAC TCA TCT CAt gtt gac aat taa tca tcg gca t-3′ | |
| MCMVΔm42 | 5′-ATA GAG GAC GTG GTT ATG CTA CTT TTG TGA ACG GAC GCG GTT GGG AGG ACg aat tca gtc ctg ctc ctc ctc ggc ca-3′ |
| 5′-AGA ACG AGT ATG ACC AAG AGG GAC ACA CAG ACT CCG ACC GTG ATG GCG GTt gtt gac aat taa tca tcg gca t-3′ | |
| MCMVΔM45 | 5′-GCT AGA GAA GTT CTA CGT CGA CGT CGG GCC CCT CGT CGA GTT CGC GTG ACg aat tca gtc ctg ctc ctc ggc ca-3′ |
| 5′-TCG TGC AGG CGA TGG GGC TCG ATC TTG ACG GAG CGC ACG CAC TCA TCG AGt gtt gac aat taa tca tcg gca t-3′ | |
| AD169ΔUL37x1 | 5′-TTT CAA GAC GAC GTG AGA CCC ACA CGC GGG TTT CAC TTC TTT CTT TAA Tta ccc ata cga cgt ccc ag-3′ |
| 5′-TTT CTT AAC CAA GGC GGG AGA GGA TCT TCA AGG CGT TTT CGC TGG ATC Cag gac gac gac gac aag taa-3′ | |
| MCMV-m41HA | 5′-TCC TCG CAG TGA TCT GCA TCG TCA TCC TGA TCG TCG TGA TCA TTG ACA GAt acc cat acg acg tcc cag-3′ |
| 5′-AGT CAT CCG ATG ATC GTG TCG CCG CCC GAC CGC CCT CCT CCC CCA ATT CAa gga cga cga cga caa gta a-3′ | |
| AD169-UL37x1HA | 5′-GGC AAC GAG CTC GGA TGC TGC AGC ACA ACG GCC CCC AGC AGT CTC ACC AGt acc cat acg acg tcc cag-3′ |
| 5′-CCT TCT TAT ACT ATC CCG GAG TCT GTG GTT TTT TTG TTT ACC CCT GCT TAa gga cga cga cga caa gta a-3′ |
a
The HA tag is in capital letters, and restriction sites are in italics.
b
Homologous sequences are in capital letters, and introduced EcoRI sites are in italics.