FIG. 6.

Receptor specificity and efficiency of transduction by a CD40-targeted Ad vector. CD40-negative 293 cells and CD40-positive 293.CD40 cells were transduced with Ad5LucFF/CD40L or untargeted Ad5Luc1 (control) at an MOI of 10 viral particles/cell. Prior to infection, the cells in the samples shown by the gray and white bars were preincubated with the recombinant Ad5 fiber knob and with sCD40L (each used at 100 μg/ml), respectively. Black bars correspond to luciferase activity in cells infected in the absence of inhibitors. Luciferase activity (relative light units) detected in the lysates of infected cells is the average of three measurements. Error bars indicate standard deviations.