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Integration of mutant F5Δ and FF/CD40L proteins into mosaic Ad5Luc.FF/CD40L.F5Δ virions. Aliquots of 1010 viral particles of Ad5Luc1 (lane 1), Ad5Luc.FF/CD40L (lane 2), and Ad5Luc.FF/CD40L.F5Δ (lane 3) were fully denatured, and viral proteins were separated by SDS-PAGE. The wild-type (F5) and mutant (F5Δ) Ad5 fibers as well as the FF/CD40L chimera were identified on the blots with MAb 4D2.
