FIG. 1.
Coimmunoprecipitation assay with anti-UL11 serum. Vero cells infected with HSV-1 KOS for 5 h were metabolically labeled with [35S]methionine-cysteine for 3 h and harvested with NP-40 lysis buffer. The UL11 protein (indicated by a bracket) was immunoprecipitated with anti-UL11 serum, separated by SDS-PAGE, and visualized by autoradiography. Specific coimmunoprecipitated proteins are indicated by arrowheads (40 kDa) and asterisks (31, 34, and 37 kDa). Plus and minus signs denote HSV-1-infected cells and noninfected cells, respectively. (A) Rabbit anti-UL11 serum Rb72. (B) Rabbit anti-UL11 serum Rb73.