FIG. 3.

Analysis of UL11 homologs. (A) Sequence alignment of UL11 homologs from HSV-1, MDV, and PRV. Amino acids that are identical are indicated by asterisks, whereas residues that are conserved are denoted by a period (lower conservation) or a colon (higher conservation). The locations of the dileucine motif and the acidic cluster within HSV-1 UL11 are indicated by solid lines. (B) Subcellular localization. A7 cells expressing UL11-GFP chimeras were visualized 18 h posttransfection by confocal microscopy. (C) GST pull-down assay with HSV-1-infected cell lysates. Purified GST-UL11 fusion proteins were incubated with radiolabeled, HSV-1-infected cell lysates. Bound proteins were separated by SDS-10% PAGE and detected by autoradiography. The arrowhead denotes the position of the 40-kDa protein. (D) GST pull-down assay with PRV-infected cell lysates. The assay was performed as described for panel C, except that GST fusion proteins were incubated with either HSV-1- or PRV-infected cell lysates.