FIG. 5.

Association of UL11 and UL16 within transfected cells. (A) GST pull-down assay with transfected-cell lysates. A7 cells expressing a UL16-GFP fusion were harvested in NP-40 lysis buffer at 24 h posttransfection. After the lysates were precleared for 2 h with glutathione-Sepharose beads, purified GST-UL11 fusion proteins were added. Proteins bound to the GST constructs were separated by SDS-9% PAGE and electrotransferred to nitrocellulose membranes. Western blot analysis was then performed with a GFP-specific antiserum. (B) Subcellular localization of UL16-GFP in the presence or absence of UL11. A7 cells were transfected with the indicated constructs and visualized 18 h later by live-cell confocal microscopy.