TABLE 2.

Quantitative analysis of VZV gene transcription^a

A1 clone	A2 ORF	A3 region	A4 column	A5 row	1 dpi			2 dpi			3 dpi			4 dpi
					B1 rel exp	B2 std	B3 order	C1 rel exp	C2 std	C3 order	D1 rel exp	D2 std	D3 order	E1 rel exp	E2 std	E3 order
1	1	all	1	1	3.6	0.7	36	19.3	3.8	39	62.5	8.9	24	36.0	1.5	19
2	2	5′	1	2	3.7	0.7	25	28.2	0.6	22	71.9	6.6	19	38.0	0.7	17
3	2	3′	1	3	3.4	0.5	56	13.2	0.3	55	26.5	1.0	47	13.1	0.4	43
4	3	5′	1	4	3.5	0.2	49	12.8	0.4	59	19.3	0.6	59	15.0	0.6	41
5	3	3′	1	5	4.1	0.7	8	25.5	1.2	28	51.3	4.9	29	39.8	2.4	15
6	4	5′	1	6	2.7	0.1	117	5.7	0.9	119	7.8	0.4	118	5.3	0.1	116
7	4	3′	1	7	2.7	0.1	122	21.8	0.3	34	42.7	3.8	36	19.2	0.8	33
8	5	5′	1	8	1.5	0.7	147	2.5	0.5	148	4.8	0.0	141	1.9	0.1	150
9	5	3′	1	9	0.3	0.1	152	0.4	0.1	152	0.5	0.0	152	0.5	0.0	152
10	6	5′	2	1	3.3	0.2	59	10.8	0.3	68	11.4	0.1	91	5.5	0.1	114
11	6	3′	2	2	3.6	0.3	35	9.4	0.7	77	12.1	0.2	85	6.8	0.3	90
12	7	5′	2	3	3.5	0.3	46	9.7	1.2	74	17.3	0.1	69	8.8	0.1	70
13	7	3′	2	4	4.5	0.3	5	14.0	3.2	51	26.6	1.2	46	14.8	1.7	42
14	8	5′	2	5	3.2	0.6	81	6.1	0.3	111	8.6	0.0	106	5.8	0.0	110
15	8	3′	2	6	3.1	0.2	96	9.3	1.3	79	8.5	0.0	107	6.7	0.1	91
16	9	5′	2	7	2.9	0.7	109	46.5	1.0	11	164.5	35.3	8	114.3	2.3	1
17	9	3′	2	8	10.8	0.7	1	179.5	20.7	1	321.0	2.7	1	93.9	9.0	2
18	10	5′	2	9	1.7	0.3	145	2.4	0.4	149	3.8	0.5	145	2.5	0.2	146
19	10	3′	3	1	4.0	1.5	11	28.8	1.9	21	63.3	0.7	22	26.1	0.3	24
20	11	5′	3	2	4.4	1.0	6	24.6	4.7	30	82.3	2.2	17	25.3	0.7	25
21	11	3′	3	3	3.7	0.5	30	31.4	4.9	17	93.8	13.9	13	29.8	0.2	22
22	12	5′	3	4	3.3	0.2	63	6.0	2.0	112	8.2	0.3	113	6.0	0.2	102
23	12	3′	3	5	3.2	0.1	87	5.4	1.5	126	5.8	0.3	137	4.8	0.0	124
24	13	5′	3	6	3.2	0.9	89	9.3	1.3	78	14.3	0.0	78	10.0	0.3	56
25	13	3′	3	7	3.2	0.6	82	14.6	2.4	49	27.8	0.5	44	15.3	0.1	40
26	14	5′	3	8	2.4	0.3	130	2.7	0.0	145	3.2	0.3	148	2.5	0.2	147
27	14	3′	3	9	2.4	0.2	131	2.6	0.0	146	2.4	0.2	149	2.4	0.2	148
28	15	5′	4	1	3.8	0.8	23	17.5	1.9	44	36.1	3.9	39	18.7	0.5	36
29	15	3′	4	2	3.3	0.8	61	12.1	3.1	64	27.4	3.6	45	11.5	0.2	48
30	16	5′	4	3	3.3	0.3	67	6.4	1.5	106	6.9	0.2	126	3.9	0.0	137
31	16	3′	4	4	3.3	0.1	74	5.5	1.5	122	6.7	0.0	132	5.1	0.0	119
32	17	5′	4	5	3.3	0.0	66	7.0	1.5	94	7.3	0.0	124	5.3	0.0	117
33	17	3′	4	6	3.3	0.2	69	8.9	2.9	82	11.2	0.3	94	6.9	0.1	89
34	18	5′	4	7	2.8	0.1	114	8.9	1.0	84	18.0	2.4	64	7.9	0.2	76
35	18	3′	4	8	3.7	0.3	28	15.4	0.5	48	24.8	2.7	52	9.4	0.4	60
36	19	5′	4	9	2.4	0.3	128	3.8	0.8	138	5.7	0.4	138	3.9	0.4	136
37	19	3′	5	1	3.7	0.0	26	9.6	1.1	76	15.2	0.5	76	7.5	0.1	80
38	20	5′	5	2	3.4	0.1	52	21.6	7.2	35	48.7	2.3	31	15.9	0.1	39
39	20	3′	5	3	3.7	0.4	29	30.7	6.1	19	69.2	8.2	20	21.5	0.6	29
40	21	5′	5	4	3.2	0.1	90	5.8	2.5	117	6.6	0.2	133	4.3	0.1	133
41	21	3′	5	5	3.6	0.2	39	13.8	2.7	52	21.1	0.0	56	8.7	0.0	71
42	22	5′	5	6	3.0	0.2	103	13.3	4.4	54	16.9	0.9	72	8.8	0.1	69
43	22	3′	5	7	2.5	0.0	126	10.4	1.4	70	18.5	1.5	61	7.1	0.0	85
44	23	5′	5	8	0.9	0.2	149	6.0	0.0	114	8.3	0.6	111	6.6	0.3	92
45	23	3′	5	9	0.4	0.1	150	5.7	0.8	118	12.2	1.6	83	3.3	0.2	143
46	24	5′	6	1	3.6	0.2	40	25.0	3.9	29	61.0	5.6	25	33.4	1.4	20
47	24	3′	6	2	3.8	0.1	20	26.1	3.0	26	87.2	2.0	15	41.2	2.4	14
48	25	5′	6	3	3.7	0.3	32	12.3	2.6	63	19.4	0.9	58	9.2	0.1	65
49	25	3′	6	4	3.0	0.4	101	5.3	0.7	128	6.8	0.4	129	4.3	0.1	132
50	26	5′	6	5	3.4	0.7	58	13.8	2.6	53	17.0	0.8	71	7.8	0.1	78
51	26	3′	6	6	3.1	0.2	94	12.9	2.2	58	19.4	0.0	57	8.9	0.1	68
52	27	5′	6	7	2.3	0.1	133	9.0	3.0	81	16.0	0.4	74	7.4	0.4	82
53	27	3′	6	8	1.9	0.1	143	9.7	1.6	75	25.5	0.5	49	9.4	0.4	61
54	28	5′	6	9	1.8	0.2	144	7.3	0.6	93	17.5	0.1	68	6.0	0.7	101
55	28	3′	7	1	3.8	0.3	17	27.6	2.2	23	56.1	7.1	27	20.8	0.8	32
56	29	5′	7	2	3.5	0.7	45	17.4	0.5	45	25.3	0.7	51	12.7	0.0	44
57	29	3′	7	3	3.4	0.4	50	18.5	0.1	41	34.4	2.0	40	11.2	0.1	51
58	30	5′	7	4	3.3	0.1	75	5.9	1.6	115	7.3	0.0	123	4.7	0.0	126
59	30	3′	7	5	3.2	0.0	80	10.8	2.1	69	12.3	0.1	82	9.9	0.2	57
60	31	5′	7	6	2.8	0.4	111	6.7	1.9	100	11.7	0.1	88	5.9	0.2	105
61	31	3′	7	7	2.6	0.2	124	12.5	3.5	61	18.3	1.1	62	9.4	0.5	64
62	32	5′	7	8	1.9	0.1	142	8.9	2.9	83	17.6	0.4	66	9.4	0.4	63
63	32	3′	7	9	3.1	0.3	92	13.0	1.6	57	38.4	0.3	38	12.3	0.2	45
64	33	5′	8	1	4.0	0.8	14	53.7	0.5	10	192.9	22.6	5	55.6	2.2	9
65	33	3′	8	2	4.7	1.5	4	78.7	11.2	3	222.9	39.4	3	68.3	2.9	7
66	34	5′	8	3	3.4	0.2	51	10.0	0.6	72	11.8	0.9	87	5.8	0.0	108
67	34	3′	8	4	3.3	0.1	71	8.1	0.2	90	11.9	0.6	86	5.8	0.3	109
68	35	5′	8	5	2.8	0.2	112	5.4	1.0	127	8.2	0.2	112	4.5	0.2	130
69	35	3′	8	6	4.0	1.8	13	5.9	1.6	116	9.9	0.1	100	6.0	0.2	103
70	36	5′	8	7	2.7	0.7	119	4.8	2.2	135	7.4	0.4	122	4.6	0.2	128
71	36	3′	8	8	2.6	0.3	123	12.1	2.4	65	22.6	1.5	54	8.1	0.8	74
72	37	5′	8	9	2.4	0.3	129	3.8	0.9	140	5.0	0.0	140	3.9	0.3	135
73	37	3′	9	1	4.1	0.3	10	31.0	5.0	18	55.4	8.9	28	16.9	0.9	38
74	38	5′	9	2	3.2	0.5	76	17.9	3.6	42	50.3	2.7	30	27.1	1.7	23
75	38	3′	9	3	3.2	0.2	85	38.8	0.8	13	87.0	10.0	16	36.5	1.7	18
76	39	5′	9	4	3.0	0.1	102	6.2	1.6	110	9.2	0.4	102	5.9	0.2	106
77	39	3′	9	5	3.2	0.5	78	11.3	2.8	66	11.6	1.2	90	7.4	0.3	81
78	40	5′	9	6	3.1	0.4	97	6.2	1.5	109	8.3	0.2	110	5.7	0.3	111
79	40	3′	9	7	2.9	0.4	108	5.0	1.3	134	8.5	0.1	108	5.6	0.5	113
80	41	5′	9	8	2.1	0.3	138	6.7	1.4	102	17.8	0.1	65	11.3	0.7	50
81	41	3′	9	9	1.6	0.3	146	17.3	4.2	46	28.6	5.9	43	10.2	0.9	55
82	42	5′	9	10	0.3	0.0	151	0.5	0.1	151	1.0	0.0	151	0.5	0.0	151
83	42	3′	10	1	3.2	0.6	84	20.8	1.2	37	39.1	3.8	37	10.8	0.1	54
84	43	5′	10	2	3.3	0.2	62	15.5	2.2	47	17.3	1.6	70	7.0	0.1	88
85	43	3′	10	3	2.7	0.2	120	12.4	2.2	62	8.0	0.8	115	4.2	0.2	134
86	44	5′	10	4	3.0	0.2	104	14.1	2.6	50	11.2	1.0	95	5.9	0.3	107
87	44	3′	10	5	3.5	0.3	47	28.8	11.1	20	47.9	4.8	32	23.5	1.8	27
88	45	5′	10	6	3.1	0.6	93	6.4	3.0	104	8.4	0.6	109	5.6	0.3	112
89	45	3′	10	7	2.8	0.4	113	5.0	2.3	131	7.6	0.6	120	4.8	0.3	125
90	46	5′	10	8	2.9	0.7	105	5.5	2.9	124	7.9	0.6	116	5.1	0.2	122
91	46	3′	10	9	2.6	0.5	125	3.8	1.6	139	4.5	0.2	143	3.8	0.3	141
92	47	5′	10	10	2.1	0.6	136	2.6	1.1	147	3.7	0.1	146	3.0	0.2	144
93	47	3′	11	1	3.2	1.1	83	19.8	3.2	38	6.7	0.4	131	5.0	0.1	123
94	48	5′	11	2	3.4	1.3	57	18.7	7.0	40	18.2	1.5	63	12.2	0.4	46
95	48	3′	11	3	3.9	0.6	15	54.4	7.0	9	130.8	36.3	10	54.3	2.6	10
96	49	all	11	4	5.9	0.7	3	69.5	25.1	5	152.1	31.9	9	70.5	13.8	6
97	50	5′	11	5	3.6	0.9	37	25.9	11.8	27	56.2	10.4	26	25.0	1.2	26
98	50	3′	11	6	3.3	1.1	60	57.4	26.6	8	112.0	6.7	11	43.2	1.9	13
99	51	5′	11	7	3.7	0.5	31	24.1	14.6	31	44.1	4.5	35	21.5	0.9	30
100	51	3′	11	8	2.9	0.6	107	5.6	3.4	121	6.7	0.6	130	5.1	0.2	121
101	52	5′	11	9	2.7	0.9	116	6.3	3.4	107	7.0	0.8	125	5.9	0.0	104
102	52	3′	11	10	2.3	0.6	132	5.2	2.6	129	6.8	0.4	127	4.4	0.4	131
103	53	5′	12	1	3.4	1.2	54	21.9	3.1	33	21.3	0.6	55	11.4	0.2	49
104	53	3′	12	2	3.4	1.2	55	33.5	4.7	16	30.1	2.9	42	17.8	0.5	37
105	54	5′	12	3	3.8	1.3	19	20.9	5.5	36	45.6	4.2	34	23.4	0.1	28
106	54	3′	12	4	3.1	0.8	91	6.9	2.4	96	14.1	0.4	79	9.1	0.1	67
107	55	5′	12	5	2.9	0.4	106	6.7	3.3	98	8.9	0.6	105	5.1	0.3	120
108	55	3′	12	6	3.6	0.8	41	8.5	4.4	85	11.3	1.2	93	6.5	0.2	93
109	56	5′	12	7	3.3	0.4	68	6.7	3.7	99	8.9	0.4	104	6.2	0.3	97
110	56	3′	12	8	3.1	0.2	100	8.4	5.8	87	11.0	0.7	96	9.9	0.0	58
111	57	all	12	9	4.0	1.5	12	40.0	25.4	12	90.4	8.2	14	52.0	2.6	11
112	58	5′	12	10	2.1	0.6	139	11.2	5.5	67	25.6	1.4	48	9.4	0.3	62
113	58	3′	13	1	3.8	1.5	18	38.5	2.8	14	107.0	0.6	12	39.0	0.2	16
114	59	5′	13	2	2.8	0.9	115	12.7	6.7	60	11.7	1.1	89	6.1	0.1	99
115	59	3′	13	3	3.6	1.4	34	13.1	3.2	56	25.5	2.3	50	11.0	0.1	53
116	60	5′	13	4	3.3	1.4	65	9.9	3.4	73	14.7	0.5	77	8.3	0.2	72
117	60	3′	13	5	3.3	1.4	72	10.1	3.6	71	17.6	0.8	67	7.9	0.5	75
118	Int 6	na	13	6	3.2	0.0	86	7.3	2.9	92	10.8	0.8	97	7.1	0.3	84
119	Int 7	na	13	7	3.5	0.1	48	6.9	3.4	97	8.1	1.2	114	6.3	0.3	96
120	Int 8	na	13	8	2.5	0.2	127	3.5	1.9	142	4.6	0.3	142	3.8	0.2	139
121	Int 9	na	13	9	3.2	0.8	79	8.4	5.2	86	12.6	0.7	81	9.8	0.6	59
122	61	5′	13	10	1.0	0.5	148	2.9	1.5	144	6.8	0.0	128	2.8	0.0	145
123	61	3′	14	1	3.3	1.4	73	26.5	1.5	25	64.4	4.4	21	45.1	0.7	12
124	Int 10	na	14	2	3.5	1.0	42	17.7	6.5	43	23.4	1.1	53	11.1	0.3	52
125	Int 11	na	14	3	3.9	0.9	16	5.5	2.5	123	7.8	0.1	117	4.6	0.0	127
126	Int 12	na	14	4	3.5	0.5	44	3.7	0.7	141	3.5	0.1	147	3.5	0.1	142
127	62	5′-a	14	5	2.7	0.5	121	4.6	1.9	136	6.4	0.3	134	4.5	0.0	129
128	62	5′-b	14	6	3.2	0.7	77	6.0	2.1	113	10.4	0.0	98	7.1	0.2	86
129	62	5′-c	14	7	3.1	0.1	95	5.0	1.7	132	5.9	0.2	136	6.3	0.1	95
130	62	5′-d	14	8	2.7	0.0	118	5.0	1.5	133	5.6	0.1	139	5.2	0.2	118
131	62	5′-e	14	9	2.8	0.5	110	9.0	3.6	80	16.4	0.2	73	12.1	0.4	47
132	62	5′-f	14	10	2.0	0.8	141	6.4	1.6	105	12.1	0.8	84	8.2	0.5	73
133	62	5′-g	15	1	2.3	0.9	135	8.3	0.7	88	12.7	0.6	80	6.1	0.3	100
134	62	5′-h	15	2	3.6	1.1	38	8.0	0.0	91	10.3	0.2	99	6.2	1.0	98
135	62	5′-i	15	3	3.8	0.2	22	4.0	0.8	137	4.0	0.2	144	3.9	0.1	138
136	62	3′	15	4	3.1	0.7	99	71.6	14.4	4	174.9	5.7	6	60.4	1.0	8
137	Int 1	na	15	5	4.1	0.3	9	23.6	12.9	32	47.0	1.0	33	21.4	0.7	31
138	Int 2	na	15	6	3.3	0.3	70	6.7	3.3	101	11.4	0.1	92	7.8	0.0	79
139	Int 3	na	15	7	2.3	0.2	134	2.0	0.2	150	1.9	0.0	150	2.4	0.0	149
140	Int 4	na	15	8	2.1	0.4	137	3.4	1.2	143	6.3	0.9	135	3.8	0.3	140
141	Int 5	na	15	9	3.1	0.5	98	5.2	1.6	130	9.3	0.4	101	7.9	0.0	77
142	63	5′	15	10	2.0	0.4	140	6.6	4.2	103	30.4	0.1	41	19.0	0.4	34
143	63	3′	16	1	3.7	1.7	33	64.4	15.7	7	194.3	3.7	4	91.2	6.4	3
144	64	5′	16	2	3.8	1.2	24	69.0	11.2	6	165.0	8.2	7	74.3	4.0	5
145	64	3′	16	3	4.3	1.4	7	100.4	31.8	2	236.0	31.8	2	83.7	2.3	4
146	65	all	16	4	3.8	0.5	21	8.1	3.0	89	15.2	1.9	75	9.2	0.0	66
147	66	5′	16	5	3.7	0.2	27	6.3	2.8	108	7.7	0.7	119	5.5	0.2	115
148	66	3′	16	6	3.5	0.1	43	5.6	3.0	120	8.9	0.3	103	6.4	0.1	94
149	67	5′	16	7	3.4	0.4	53	7.0	4.7	95	19.0	0.2	60	7.3	0.2	83
150	67	3′	16	8	6.4	2.4	2	26.6	19.7	24	62.5	8.4	23	18.8	0.2	35
151	68	5′	16	9	3.2	0.4	88	5.5	3.3	125	7.5	0.4	121	7.0	0.3	87
152	68	3′	16	10	3.3	0.3	64	34.4	23.7	15	76.8	6.4	18	33.0	0.8	21
Array controls
Avg plasmid DNA					2.3	0.2	132	3.3	0.3	144	4.1	0.2	144	4.7	0.1	126
Avg no DNA					1.0	0.1	149	1.0	0.0	152	1.0	0.1	152	1.0	0.1	152
Avg actin					0.1	0.0	155	0.6	0.0	153	0.2	0.0	155	0.2	0.0	155
Avg GAPdH					0.1	0.0	156	0.0	0.0	156	0.0	0.0	156	0.1	0.0	156

^aVZV open reading frame region [5′, 3′, or entire (all)] was PCR amplified and inserted into pGEM. Target DNA array coordinates (column and row) are given. rel exp, relative expression of the individual target region. std, standard deviation of mean relative expression. order, order (in decreasing magnitude) of the individual target relative expression. dpi, day postinfection.