FIG. 1.

Hendra virus V protein inhibits IFN signaling. (A) Amino acid sequence alignment of Nipah and Hendra virus V proteins. (B) Human fibrosarcoma 2fTGH cells were transfected with expression vectors for HA-tagged Nipah virus V (NiV), Hendra virus V (HeV), or HA epitope tag expression vector with no cDNA insert. Cells were also cotransfected with either the IFN-α-inducible ISRE-luciferase reporter gene or an IFN-γ-inducible GAS-luciferase reporter gene. Cells were treated with (+) or without (−) 1,000 U of IFN-α per ml or 5 ng of IFN-γ per ml 12 h prior to lysis and luciferase assays. All bars represent average values from triplicate samples normalized to those obtained with cotransfected renilla luciferase ± standard deviations and are expressed as percentages of values for IFN-stimulated controls.