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. 2003 Nov;77(21):11842–11845. doi: 10.1128/JVI.77.21.11842-11845.2003

FIG. 2.

FIG. 2.

Hendra virus V forms STAT1- and STAT2-containing complexes. (A, left panel) 293T cells were transfected with either FLAG epitope expression vector with no cDNA insert (lane C), FLAG-tagged Hendra virus V expression vector (lane H), or FLAG-tagged Nipah virus V expression vector (lane N). Whole-cell extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and processed for immunoblotting with antiserum to STAT1, STAT2, STAT3 (all from Santa Cruz Biotechnology), or FLAG (Sigma). Positions of prestained molecular weight standards and STAT1, STAT2, STAT3, and FLAG Henipavirus V proteins are indicated. (A, right panel) Extracts from the left panel were immunoprecipitated (IP) by using an M2 FLAG affinity gel (Sigma) and analyzed by immunoblotting. (B) Cell extracts of 293T cells transfected with expression vectors for FLAG-tagged Nipah virus V (NiV), Hendra virus V (HeV), or green fluorescent protein (GFP) were separated by chromatography on a Superdex-200 column (Pharmacia). Positions of STAT1 and STAT2 were determined by Western blotting (WB) every other fraction. The column was calibrated with high- and low-molecular-weight calibration kits (Pharmacia).