Incubation of linearized substrate with proteins from the HSV-AAV replication complex. Products were separated on a 0.8% agarose gel and visualized by SYBR green staining. Shown are the addition of Rep 68 and the products of the HSV UL29, UL 30, and UL42 genes. S, starting substrate; A, AAV component of the substrate; V, vector component of the substrate; H, putative hybrid double- and single-stranded species. Shown on the right are DNA size markers (SM).