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. 2003 Nov;77(21):11480–11490. doi: 10.1128/JVI.77.21.11480-11490.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 8. — Deleted ITRs result in greater amounts of Rep-only rescue. Shown are graphs from SYBR green staining of 0.8% agarose gels in which assay products were separated. In each case, higher-molecular-weight material is to the left and lower-molecular-weight material is to the right. Substrates were incubated with Rep for 4 h (HSV proteins omitted) and then separated by electrophoresis on agarose gels. L0, left ITR with no missing bases; L-22, left ITR with terminal 22 bases missing; R0, right ITR with no bases missing; R-8, right ITR with 8 terminal bases missing. S, full-length substrate; V, vector component; A, AAV component.