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. 2003 Nov;77(21):11859–11866. doi: 10.1128/JVI.77.21.11859-11866.2003

FIG. 3.

FIG. 3.

CS-specific CD8+ cellular immune responses induced in mice primed with CA-CS and boosted 2 weeks later with VAC-CS or MVA-CS viruses. (A) On days 5, 14, and 21 after boosting, peripheral blood mononuclear cells from mice primed with CA-CS and boosted with either VAC-CS or MVA-CS were analyzed by flow cytometry by staining with H-2d tetramer-phycoerythrin and anti-CD8-FITC. The percentages of double-positive cells are presented. The results represent those obtained in one of two identical experiments and are means ± standard deviations for 10 mice. (B) Long persistence of CS-specific IFN-γ-secreting CD8+ cells after priming-boosting immunization. CS-specific CD8+-T-cell responses in the spleen were measured by ELISPOT assay at 2 weeks and at 1, 2, and 4 months after priming with CA-CS and boosting with MVA-CS or VAC-CS. The results are those obtained in one of two identical experiments and are means ± standard deviations for three mice.