FIG. 5.

Replication of a poliovirus replicon is not inhibited by ZAP. Rat2-HA-Zeo cells expressing vector alone (shaded bars) and Rat2-NZAP-Zeo cells expressing the amino-terminal portion of ZAP fused to the product of the zeocin resistance gene (open bars) were seeded in six-well plates and transfected with 3.5 μg of PolioRep/GFP RNA encoding a GFP-expressing poliovirus replicon per well. The percentage of cells expressing GFP was determined by flow cytometry 8 h after transfection. Each bar represents the mean of results for three independent wells, with error bars indicating the standard deviations. Similar results were obtained in two independent experiments.