FIG. 5.
Bone marrow-derived DC from CD4C/HIVMutA transgenic mice express the HIV transgene and are impaired in their maturation process. Bone marrow progenitors derived from the femur of nontransgenic (non-Tg) and transgenic (Tg) mice were allowed to mature for 6 days in the presence of 10% fetal bovine serum and GM-CSF. Cells were then recultured for an additional 24 h with (b) (right) or without (a and b) (left) lipopolysaccharide (10 μg/ml). The recovered cells showed dendritic morphology and expressed CD11c, as assessed by FACS. (a) These bone marrow-derived DC were analyzed for transgene expression by in situ hybridization with an HIV-1-specific antisense probe and a sense probe as a control. Counterstaining was done with hematoxylin and eosin. Magnification, ×40; bar, 50 μm. (b) Bone marrow-derived DC were analyzed by FACS for surface expression of MHC I, MHC II, CD40, and B7-2 (CD86) molecules. The data are representative of at least four different experiments, each involving cells from the bone marrow of sex- and age-matched transgenic (n = 3) and control nontransgenic (n = 3) mice.