TABLE 1.
Expt no. | Mouse age (mo) | No. of micea | Nontransgenic
|
Transgenic
|
||||||
---|---|---|---|---|---|---|---|---|---|---|
Total no. (105) | % of DC
|
Total no. (105) | % of DC
|
|||||||
CD11bneg-int | CD11bhi | CD8α+ | CD11bneg-int | CD11bhi | CD8α+ | |||||
1 | 1.5 | 2 | 1.3 | 81 | 15 | 35 | 3.0 | 75 | 21 | 20 |
2 | 2 | 2 | 0.7 | 69 | 29 | 25 | 0.7 | 65 | 35 | 16 |
3 | 2 | 2 | 0.8 | 65 | 29 | 25 | 0.8 | 60 | 39 | 24 |
4 | 2 | 4 | 1.4 | 66 | 34 | ND | 0.8 | 59 | 41 | ND |
5 | 2.5 | 2 | 0.5 | 49 | 48 | ND | 0.5 | 38 | 60 | ND |
6 | 2.5 | 2 | 1.5 | 58 | 16 | 22 | 0.7 | 50 | 16 | 9 |
1 to 6 | 1.5-2.5 | 14 | 1.0 ± 0.2 | 65 ± 4 | 28 ± 5 | 27 ± 3 | 1.0 ± 0.4 | 58 ± 5* | 35 ± 6** | 17 ± 3 |
7 | 3 | 3 | 0.6 | 68 | 32 | ND | 0.3 | 45 | 55 | ND |
8 | 3 | 2 | 1.4 | 69 | 29 | ND | 0.5 | 24 | 75 | ND |
9 | 3 | 4 | 2.2 | 37 | 64 | ND | 1.0 | 22 | 79 | ND |
10 | 3 | 2 | 3.0 | 72 | 27 | 23 | 2.4 | 68 | 31 | 17 |
11 | 3.5 | 3 | 1.3 | 83 | 17 | ND | 1.5 | 72 | 28 | ND |
12 | 4 | 2 | 1.2 | 60 | 34 | ND | 1.0 | 46 | 50 | ND |
13 | 4 | 3 | 0.6 | 71 | 29 | 10 | 0.5 | 46 | 54 | 6 |
14 | 5.5 | 2 | 1.7 | 74 | 23 | ND | 1.0 | 53 | 46 | ND |
7 to 14 | 3-5.5 | 21 | 1.5 ± 0.3 | 67 ± 5 | 32 ± 5 | 17 ± 7 | 1.0 ± 0.2 | 47 ± 6* | 52 ± 6** | 12 ± 6 |
The number indicated applies to both nontransgenic and transgenic mice (i.e., 2 indicates two of each). Total, data represent absolute numbers of DC recovered per mouse. CD11b levels of expression appear as high (CD11bHi) or negative to intermediate (CD11bneg-int). The data are presented as mean ± standard error of the mean. ND, not done. Each experiment was done with sex-matched littermate controls. *, P = 0.023, **, P = 0.03 (two-way analysis of variance).