Table 2.

Fragments predicted from combined DpnI and EcoKI cutting on concatemeric D364 DNA, assuming the midpoint cleavage model for EcoKI

EcoK sites	Midpoint*	Affected DpnI fragment	Predicted fragment size, kb	Closest size fragment observed, kb
K-I → K-II	20,310	A	6.5	6.6
K-IV → K-I	6,058	C  +  B	9.8, 1.6	9.6
K-II → K-III	29,040	A	8.7	8.8
K-III → K-IV	34,468	A	5.4	5.45
K-II → K-IV	20,310	A	14.8, 6.5	14, 6.6
(not at K-III)
K-II → K-IV	31,453	A	17.7, 3.7†	17
(not at K-I, K-III)
K-II → K-I	711	F + C + B‡	7.0, 4.8	7.45, 4.8
(not at K-III, K-IV)

  

^*Nucleotide position in D364 DNA. 

^†Not resolved from the DpnI C fragment. 

^‡Assumes the GATC sequence between the F and C fragments is not methylated fully in vivo.