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. Author manuscript; available in PMC: 2009 Mar 1.

Published in final edited form as: Arch Biochem Biophys. 2007 Dec 10;471(1):32–41. doi: 10.1016/j.abb.2007.12.001

Equilibrium analytical ultracentrifugation of (A) 2Fe-BioB and (B) apoBioB. Each protein was diluted to 5 μM in 50 mM Tris HCl, 1 mM TCEP, pH 7.5, at 20°C and subjected to centrifugation at 10000, 15000, and 20000 rpm for 2-4 hrs. Five radial scans at 280 nm were averaged for each data set. Data are globally fit using Sedphat [24] to a mixture of nonequilibrating BioB dimer and tetramer.

Equilibrium analytical ultracentrifugation of (A) 2Fe-BioB and (B) apoBioB. Each protein was diluted to 5 μM in 50 mM Tris HCl, 1 mM TCEP, pH 7.5, at 20°C and subjected to centrifugation at 10000, 15000, and 20000 rpm for 2-4 hrs. Five radial scans at 280 nm were averaged for each data set. Data are globally fit using Sedphat [24] to a mixture of nonequilibrating BioB dimer and tetramer.