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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Arch Biochem Biophys. 2007 Dec 10;471(1):32–41. doi: 10.1016/j.abb.2007.12.001

Figure 3.

Figure 3

Limited proteolysis of ApoBioB, 2Fe-BioB, and 2Fe4Fe-BioB. Each protein (1 mg/ml) was incubated with trypsin (0.001% for apoBioB and 2Fe-BioB, 0.01% for 2Fe4Fe-BioB) in 25 mM Tris HCl, pH 8 at 37 °C. At intervals, samples were removed, quenched with TLCK (10 μg/ml), and analyzed by SDS polyacrylamide electrophoresis on 4-20% gradient gels visualized with Coomassie R-250 stain. Samples removed at 30 and 60 min were also subjected to mass spectrometry (supplementary data) to facilitate identification of the major fragments produced.