Table 1.
Neuropeptides detected in the nervous system and neuroendocrine organs of the American lobster Homarus americanus
| Neuropeptide Family | [M+H]+ | Sequence | FTMS | QTOF | MALDI-TOF | Tissue location |
|---|---|---|---|---|---|---|
| AST-A type | 795.40 | EPYAFGLamide | − | + | − | Br |
| 753.39 | SPYAFGLamide | − | + | − | Br | |
| 810.41 | SGPYAFGLamide | − | + | + | Br/STG | |
| 826.41 | SGPYSFGLamide | − | + | − | Br | |
| 824.43 | ASPYAFGLamide | − | + | + | Br/STG | |
| 794.42 | AGPYAFGLamide | − | + | − | Br/VNC | |
| 822.45 | VGPYAFGLamide | − | + | − | Br | |
| 854.44 | TPSYAFGLamide | − | + | + | Br/STG | |
| 814.30 | SQYTFGLamide | − | + | − | Br | |
| 841.42 | AGGAYSFGLamide | − | + | − | Br | |
| AST-B type | 1266.64 | TNWNKFQGSWamide | + | + | + | Br/PO/STG |
| Corazonin | 1369.65 | pQTFQYSRGWTNamide | + | − | − | Br/ESG |
| CCAP | 956.38 | PFCNAFTGCamide | + | + | + | Br/PO |
| CCAP precursor related peptide | 1074.53 | DIGDLLEGKD* | − | + | − | Br/VNC |
| CHH | 8577.89 | pCHH-A[pQ61−V132amide] | + | − | + | SG/PO/STG |
| 8633.20 | pCHH-B[pQ61−V132amide] | + | − | − | SG | |
| MIH | 8478.76 | pMIH [pQ61−M131] | − | − | + | STG |
| CPRP | 3604.77 | RSVEGASRMEKLLSSSNSPSSTPLGFLSQDHSVN | + | + | + | SG/ESG/PO |
| 3544.81 | RSVEGVSRMEKLLSSISPSSTPLGFLSQDHSVN | + | + | − | SG/ESG | |
| Truncated | 1199.61 | PLGFLSQDHSV | − | + | − | SG |
| CPRPs | 1313.65 | PLGFLSQDHSVN | − | + | − | SG |
| 1149.57 | RSVEGVSRME | − | + | − | SG | |
| 1362.72 | RSVEGASRMEKL | − | + | − | SG | |
| 1503.83 | RSVEGASRMEKLL | − | + | − | SG | |
| 1562.83 | RSVEGASRMEKLLS | − | + | − | SG | |
| 1590.86 | RSVEGVSRMEKLLS | − | + | − | SG | |
| 1649.86 | RSVEGASRMEKLLSS | − | + | − | SG | |
| 1576.85 | RSVEGASRMEKLLT | − | + | − | SG | |
| 1604.88 | RSVEGVSRMEKLLT | − | + | − | SG | |
| FaRPs | 695.40 | NFLRFamide | − | + | − | Br/VNC |
| 965.54 | GGRNFLRFamide | − | + | + | Br/VNC/PO/STG | |
| 995.55 | SGRNFLRFamide | + | + | + | Br/VNC/PO/STG | |
| 1022.56 | GNRNFLRFamide | − | + | + | Br/PO/STG | |
| 1023.55 | GDRNFLRFamide | − | + | + | Br/VNC/PO/STG | |
| 1147.65 | APQRNFLRFamide | − | − | + | STG | |
| 1208.63 | DQNRNFLRFamide | + | + | + | Br/VNC/PO/STG | |
| 1271.68 | pQDLDHVFLRFamide | + | + | + | Br/VNC/ESG/PO/STG | |
| 1288.68 | QDLDHVFLRFamide | + | + | + | Br/VNC/ESG/STG | |
| 887.56 | PSLRLRFamide | − | + | − | Br/VNC | |
| 1041.63 | GPPSLRLRFamide | + | + | + | Br/VNC/STG | |
| 1105.63 | SMPSLRLRFamide | + | + | + | Br/VNC/SG/PO/STG | |
| 1121.62 | SM(O)PSLRLRFamide | + | + | − | Br/VNC | |
| 1163.67 | FEPSLRLRFamide | − | + | − | VNC | |
| 1275.72 | DTSTPALRLRFamide | + | + | + | Br/VNC/STG | |
| 1069.55 | SDRNYLRFamide | − | + | − | Br | |
| 1104.61 | GAHKNYLRFamide | + | − | + | Br/PO/STG | |
| 1289.64 | GYSDRNYLRFamide | + | + | + | Br/VNC/STG | |
| 1484.66 | GGGEYDDYGHLRFamide‡ | + | − | − | VNC | |
| Orcokinin | 1198.55 | NFDEIDRSGFamide | − | + | + | Br/VNC/SG/STG |
| 1199.53 | NFDEIDRSGF | − | + | − | SG | |
| 1270.57 | NFDEIDRSGFA | + | + | − | Br/VNC/SG/ESG | |
| 1256.55 | NFDEIDRSGFG | + | + | + | Br/VNC/SG/ESG/STG | |
| 1403.62 | NFDEIDRSGFGF | + | + | + | Br/VNC/SG/ESG/STG | |
| 1502.69 | NFDEIDRSGFGFV | + | − | + | Br/VNC/SG/ESG/STG | |
| 1517.67 | NFDEIDRSGFGFN | + | − | + | Br/VNC/SG/ESG/STG | |
| 1540.68 | NFDEIDRSGFGFH | + | + | + | Br/VNC/SG/ESG/STG | |
| 1554.70 | NFDEIDRSSFGFN | + | + | + | Br/VNC/STG | |
| Orcokinin | 1474.63 | SSEDMDRLGFGFN | + | + | + | Br/VNC/SG/ESG/STG |
| related | 1490.62 | SSEDM(O)DRLGFGFN | − | + | − | Br/VNC |
| peptides | 1213.50 | SSEDMDRLGFG | − | + | + | Br/VNC/SG/STG |
| 1227.52 | SSEDMDRLGFA | + | + | − | Br/VNC/SG | |
| 904.42 | FDAFTTGFamide | − | + | − | Br | |
| 1213.53 | FDAFTTGFGHN | + | + | + | Br/VNC/SG/STG | |
| 1186.52 | FDAFTTGFGHS | + | − | − | Br/ESG | |
| 1280.66 | VYGPRDIANLY# | + | + | + | Br/VNC/SG/STG | |
| β-PDH | 1927.03 | NSELINSILGLPKVMNDAamide | − | + | − | Br/SG |
| Proctolin | 649.37 | RYLPT | − | − | + | PO |
| Pyrokinin | 618.37 | FSPRLamide | − | + | − | Br/VNC |
| RPCH | 930.47 | pQLNFSPGWamide | − | + | − | SG |
| SIFamide | 1423.78 | VYRKPPFNGSIFamide | − | + | + | Br/VNC/ESG/STG |
| 1161.65 | RKPPFNGSIFamide | − | + | − | Br | |
| Tachykinin | 950.49 | APSGFLGM(O)Ramide | − | + | − | Br/VNC |
| 980.50 | TPSGFLGM(O)Ramide | − | + | − | Br/VNC | |
| 934.49 | APSGFLGMRamide | − | + | + | Br/VNC/SG/STG | |
| 964.50 | TPSGFLGMRamide | − | + | − | Br/VNC | |
| 766.40 | SGFLGMRamide | − | + | − | Br/VNC | |
| 863.46 | PSGFLGMRamide | − | + | − | Br/VNC | |
| 992.50 | APSGFLGMRG | − | + | − | Br/VNC | |
| Other peptide | 1099.64 | DLPKVDTALK | − | + | − | Br/VNC |
| 858.54 | KPKTEKK | − | + | − | Br | |
| 1254.81 | AVLLPKKTEKK | − | + | − | Br | |
| 1363.67 | EVEEPEAPAPPAK | − | + | − | Br | |
| 1372.79 | LRVAPEEHPVLL | − | + | − | Br | |
| 1103.56 | GPSGGFNGALAR | − | + | − | Br | |
Br, brain; VNC, ventral nerve cord; ESG, eyestalk ganglia; PO, pericardial organ; SG, sinus gland; STG, stomatogastric ganglion. AST, allatostatin; CCAP, crustacean cardioactive peptide; CHH, crustacean hyperglycemic hormone; CPRP, crustacean hyperglycemic hormone precursor related peptide; FaRP, FMRFamide-related peptide; MIH, molt inhibiting hormone; PDH, pigment dispersing hormone; RPCH, red pigment concentrating hormone
Previously known H. americanus peptides are shown in black; peptides previously described from other decapods, but new to H. americanus are shown in blue; novel peptides are shown in red.
Predicted to exist in Homarus gammarus (Chung et al., 2006), but de novo sequenced here for the first time
predicted to exist in H. americanus (Dickinson et al., 2007c), but detected in neural tissue for the first time here
predicted and de novo sequenced from H. americanus simultaneously and independently in Dickinson et al. (2007a)