Figure 5.

The F-box motif is required for DNA damage-induced focus formation of Fbh1. (A) fbh1Δ cells expressing NLS-GFP-Fbh1 (wild type) and NLS-GFP-Fbh1-fb were treated as in Figure 3 with MMS (0.1%) for 2 h at 30°C and observed by fluorescence microscopy. The upper and lower panels show GFP and DAPI images, respectively. The scale bar indicates 10 μm. (B) Quantitative analysis of NLS-GFP-Fbh1 foci. Cells with Fbh1 foci were counted and divided by the total number of cells. At least 100 cells were counted per strain. The result represents the average of three independent measurements.