Skip to main content
. Author manuscript; available in PMC: 2008 May 8.
Published in final edited form as: J Immunol. 2006 Nov;177(9):6291–6300. doi: 10.4049/jimmunol.177.9.6291

Figure 5.

Figure 5

FcγR-mediated activation causes a reduction of HIV-1 retrotranscripts and of integrated proviruses in MDMs. MDMs were infected with DNAse treated HIV-1VSV-G and plated on DNP-BSA coated plates (unstimulated, US) or stimulated with ICs (S). Luciferase activity was monitored at 24, 48 and 96 h p.i. in MDM lysates (A). Early and late retrotranscription products and 2-LTR circles were analyzed by rtPCR using the appropriate primers (R-U5, U5-Gag, 2-LTR) and probes (B–D). Integrated copies were evaluated by Alu-LTR nested rtPCR. Values are means of duplicate measures at the indicated times p.i. (E). Ratios between 2-LTR circles and integrated nuclear forms of HIV-1 at 48 and 96 h p.i. as measured by rtPCR in unstimulated or IC-activated MDMs. Results from a representative experiment of three performed with MDM from different donors are shown.