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In vitro phosphorylation of HPr(His)6 and Crh(His)6. [32P]PEP and purified EI (A) or [γ-32P]ATP and a crude extract of B. subtilis GM273 (B) were incubated together with HPr(His)6 (lane 2) or Crh(His)6 (lane 3); lane 1, control without HPr(His)6 or Crh(His)6. Proteins were separated by SDS/PAGE as described. In the case of ATP-dependent phosphorylation (B), the gel was treated for 10 min with boiling 16% trichloroacetic acid prior to autoradiography.
