Figure 4.
MALDI-MS of tryptic and CNBr cleavage products of Crh(His)6 or partially phosphorylated Crh(His)6. (A) Tryptic digests of Crh(His)6. (B) Tryptic digests of partially phosphorylated Crh(His)6. (C) CNBr cleavage of Crh(His)6. (D) CNBr cleavage of partially phosphorylated Crh(His)6. The sequences of the expected tryptic (Left) and CNBr fragments (Right) are listed, and peaks exhibiting the mass calculated for one of these fragments are labeled with the corresponding number. The two peaks exhibiting a mass of 2,530 and 2,861 (peaks a and b) in B, result probably from products present in the B. subtilis crude extract used as source for HPr kinase.