Table 2.
Effect of a crh gene disruption on CCR of inositol dehydrogenase, β-xylosidase, and of a levD′–′lacZ fusion
| System tested | Sugar added to CSK | Enzyme activity in units/mg of protein*
|
||||
|---|---|---|---|---|---|---|
| QB5081 | QB5224 | QB7098 | QB7103 ptsH1 | QB7115 | ||
| crh+ptsH+ | ptsH1 | crh::aphA3 | crh::aphA3 | ccpA::spc | ||
| β-Galactosidase (levD′–′lacZ fusion) | Fructose | 300 | 415 | 380 | 540 | 600 |
| Fructose + glucose | 20 | 110 | 35 | 450 | 290 | |
| Inositol dehydrogenase | Inositol | 280 | 330 | 280 | 340 | 390 |
| Inositol + fructose | 30 | 165 | 35 | 360 | 430 | |
| Inositol + glucose | 6 | 12 | 3 | 420 | 465 | |
| Inositol + glycerol | 45 | 340 | 50 | 670 | 480 | |
| β-Xylosidase | Xylose | 3,200 | 3,270 | 4,100 | 5,630 | 5,260 |
| Xylose + fructose | 230 | 856 | 245 | 4,870 | 3,090 | |
| Xylose + glucose | 30 | 80 | 35 | 2,360 | 3,550 | |
| Xylose + glycerol | 305 | 4,570 | 350 | 10,650 | 6,760 | |
*
Enzyme activities were calculated from two independent experiments for inositol dehydrogenase and from three independent experiments for the two other enzymes. Standard deviations did not exceed ±20% for β-xylosidase and ±12% for the two other enzymes.