Figure 3.
The POU domain adopts multiple conformations on an (OCTA+)TAATGARAT site. Shown are the cleavage products generated from POU domain–DNA crosslinking to both strands of the (OCTA+)TAATGARAT site. Lanes 2 and 3, cleavage pattern generated on the upper strand. The POUH doublet may be due to an incomplete cleavage reaction. Lanes 5 and 6, cleavage pattern generated on the lower strand. Lanes 1 and 4 (A+G), purine-cleavage pattern of the strand indicated. In lanes 5 and 6, the uppermost bands represent uncleaved DNA. Brackets, arrows, and asterisk are as in Fig. 2. (Lower) Summary of positions of cleavage. Dashed lines indicate lower-yield cleavage products.