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. 1997 Aug 5;94(16):8450–8455. doi: 10.1073/pnas.94.16.8450

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Copyright © 1997, The National Academy of Sciences of the USA

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VP16 associates with multiple conformations of the Oct-1 POU domain. Shown are the results of a crosslinking interference analysis of two unique-cysteine derivatives of the POU domain, POU_S-UC54 (lanes 2–4) and POU_H-UC50 (lanes 5–7), crosslinked to the lower strand of the (OCTA⁺)TAATGARAT site. POU_S crosslinks over the 5′ ATGC (OCTA X-links) or 3′ GARAT (GARAT X-link) sequences and POU_H crosslinks (X-link) are indicated. In lanes 2–7, the uppermost bands represent uncleaved DNA. X-link (above lanes), standard crosslinking analysis of the POU domain alone to the site; POU, crosslinking pattern of the POU-domain complex after electrophoretic mobility retardation; VIC, crosslinking interference pattern of the VP16-induced complex after electrophoretic mobility retardation.