Skip to main content
. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Dec 14;1779(3):145–151. doi: 10.1016/j.bbagrm.2007.11.012

Figure 1.

Figure 1

GATA-6 negatively regulates TN-C gene expression. (a) Cell lysates from fibroblasts transduced with 5, 10, and 20 MOI of Ad-GATA-6 or 20 MOI of Ad-LacZ (control) for 48 hours were subjected to immunoblot analysis with antibodies against TN-C and GATA-6. (b) Fibroblasts were transduced with 10 MOI of Ad-GATA-6 or control virus for 24 hours, then treated with IL-4 (10 ng/ml) or TGF-β (2.5 ng/ml) for 24 hours. Samples were assayed by immunoblot analysis with antibodies against TN-C and GATA-6 or (c) quantitative RT-PCR to determine TN-C mRNA levels. One representative Western blot of four independent experiments is shown. Band intensities were quantitated by densitometric analysis and are shown relative to the level of untreated fibroblasts transduced with control virus. * P < 0.01.