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Expression of MLN64 deletion mutants in COS-1 cells. COS-1 cells were transfected with plasmids harboring the indicated MLN64 constructs in pAT4, each containing the F domain of the human estrogen receptor in the N terminus to tag the expressed protein so that it could be recognized by a monoclonal antibody to the F domain. The numbers in parentheses refer to the amino acid residues of MLN64 encoded by the construct.
