Figure 2.

(A) Screening of ³⁵S-labeled human general transcription factors for binding to AR_142–485. The measured radioactivity is plotted relative to wells containing BSA only, which was set at 1. The binding of TBP to the C-terminal transactivation domain of the herpes simplex viral activator protein VP16 is shown for comparison. The results represent the mean ± SD of at least four observations from two or more independent experiments, except TFIIH, subunits where the mean of two observations only is shown. (B) SDS/PAGE analysis of the bound RAP74 subunit of TFIIF to AR_142–485. The input represents 5% of the material incubated per well.