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The chorismate mutases with amino acid replacements Arg16Ala, Arg157Ala, Lys168Ala, Thr242Gly, and Glu246Ala, which exhibit no in vitro activity are properly expressed in yeast. The immunoblot shows 20 μg of crude extracts of yeast strain RH2185 harboring the different ARO7 alleles on the high copy number plasmid YEp352. The protein was hybridized with polyclonal rabbit antibody raised against purified chorismate mutase.
