Table 1.

Kinetic parameters of wild-type and mutant chorismate mutases

Protein	Value of protein with amino acid replacement
	Inhibited (100 μM tyrosine)				Unliganded				Activated (10 μM tryptophan)
	kcat, s−1	Km, S0.5, mM	nH	kcat/Km, mM−1⋅s−1	kcat, s−1	Km, S0.5, mM	nH	kcat/Km, mM−1⋅s−1	kcat, s−1	Km, S0.5, mM	nH	kcat/Km, mM−1⋅s−1
Wild type	387	11.8*	1.4	32.8	360	3.8	1.6	94.7	348	0.4	1.1†	870.0
Asn194Asp	282	4.4	1.77	64.1	305	3.3	1.4	92.4	256	0.5	1.0†	512.0
Glu246Gln	52	0.15	1.1†	346.7	50	0.13	0.92†	384.6	46	0.06	0.85†	766.7

Values for k_cat, K_m, S_0.5 were determined by fitting initial velocity data to equations describing hyperbolic or cooperative saturation, respectively. Hill coefficients (n_H) were calculated from Hill plots by linear regression. 

^*Values resulted in uncertainty intervals >10% from the fitting procedure. 

^†Linear Eadie–Hofstee plots indicated hyperbolic saturation.