Figure 3.

Purity of the expressed N-terminal polypeptide and its ability to bind calcium. (A) Coomassie brilliant blue stained SDS/15% PAGE. Shown is E. coli lysate before (lane 1) and after induction (lane 2) with isopropyl β-d-thiogalactopyranoside. In lanes 3 and 4, 2 and 5 μg of purified fragments were loaded. (B) Autoradiograph of the proteins as shown in A transferred on a polyvinylidene difluoride membrane and labeled with ⁴⁵Ca²⁺ as described.