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. 1997 Aug 5;94(16):8527–8532. doi: 10.1073/pnas.94.16.8527

Figure 2.

Figure 2

Hr interacts with TR. (A) Positives isolated in a two-hybrid screen were assayed for β-galactosidase activity. Yeast strains carrying the indicated plasmids were tested. LexA-hr (bait) only, 2H11 (TRα2-VP16) only, LexA-hr + 2H11, LexA-lamin + 2H11. Results are representative of at least three individual transformants. 2H11 encodes TRα2 (amino acids 14–490) (29). TRα1 is a functional TR, whereas TRα2, which has a divergent C terminus, lacks the ability to bind thyroid hormone (29, 30); the preference for isolating TRα2 is likely because the mRNA for TRα2 is more abundant than that for TRα1 (31, 32). (B) Effect of hormone on interaction of Hr and TR. Solution β-galactosidase assay for yeast-carrying LexA-hr and TRα1-VP16 (Left) and TRα2-VP16 (Right) in the presence (+) and absence (−) of thyroid hormone. Open bars, − thyroid hormone; solid bars, + thyroid hormone.