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. 1999 Oct 26;96(22):12559–12564. doi: 10.1073/pnas.96.22.12559

Figure 1.

Figure 1

Purification of GST-β1-AR third intracellular loop binding protein(s) from bovine brain extracts. A GST pull-down assay was performed, as described in Materials and Methods, by using the GST-β1-AR third intracellular loop (A) and GST-β2-AR third intracellular loop (B), respectively. For each incubation condition, 5, 10, and 25 μg of proteins was loaded on SDS/PAGE, stained with Coomassie blue, and dried. A 40-kDa protein, as indicated by an arrow, was specifically recovered after GST-β1-AR third intracellular loop incubation with bovine brain extracts.