Fig. 5.

P25-P2C-HPV vaccination protects mice from cutaneous challenge with HPV16 and HPV45. (a–c) BALB/c mice were vaccinated s.c. three times with saline, HPV16 L2 17–36 peptide, P25 peptide, HPV45 L1 VLP, HPV16 L1 VLP, or P25-P2C-HPV and challenged on their belly with HPV16 (a and b) or HPV45 (c) pseudovirions carrying a luciferase reporter 2 weeks after the third immunization (week 10). To detect pseudoinfection, the mice were injected with luciferin 3 days after viral challenge and imaged for bioluminescence by using the IVIS 200 imaging system [see a for HPV16 challenge and supporting information (SI) Fig. S1 for HPV45 challenge]. The bioluminescence was quantified in relative light units by using Living Image 2.20 software for HPV16 challenge (b) or HPV45 challenge (c). rlu, relative light units.