Figure 4.

PCR amplification of rbcL (cpDNA), cox I (mtDNA), and aadA (bacterial sequence introduced into the cpDNA of strain L03c) genes from 10⁵ gametes of 137c (wild type) mt⁺, 137c mt⁻, L03c mt⁻, and L03c mt⁻ gametes with the outer primer pairs (A). Nested PCR amplification of rbcL, cox I, and aadA sequences from one, three, or five gametes obtained optically and immobilized by deflagellation (B). The requisite number of gametes was isolated from the cell culture with the optical tweezers and subjected directly to nested-PCR analysis in which the rbcL, cox I, and aadA sequences were amplified by two rounds of PCR by using nested primers.