Figure 5.

Nested-PCR amplification of rbcL (cpDNA), cox I (mtDNA), and aadA sequences from one optically isolated gamete or zygote 10, 30, 90, and 120 min after zygote formation. One gamete of 137c mt⁺, 137c mt⁻, L03c mt⁺, or L03c mt⁻ (A) or one zygote resulting from the crosses L03c mt⁺ × 137c mt⁻ or 137c mt⁺ × L03c mt⁻ (B) was isolated by using the optical tweezers and subjected immediately to nested PCR analysis. One typical zygote was isolated from each of the cell cultures 10, 30, 90, and 120 min after mating. To ensure reproducibility, each experiment was repeated five times (I– V). The lanes marked M are loaded with marker.