Figure 4.
Effects of intracellular calcium chelation by BAPTA on C2-ceramide- vs. bradykinin-activated NO production. BAEC were incubated with the NO indicator dye DAF-2/DA and processed for intracellular NO generation as described in the text. The integrated fluorescence intensity was measured before and 15 min after addition of bradykinin (1 μM) or C2-ceramide (5 μM); data shown are normalized to the fluorescence intensity measured prior to drug addition. As indicated in the figure, some cultures were incubated in the presence of the intracellular calcium chelator BAPTA/AM (20 μM for 20 min) prior to addition of bradykinin (BK) or C2-ceramide. For each treatment, 20–25 cells in three independent culture preparations were examined; each data point represents the mean ± SE.